›› 2011, Vol. 23 ›› Issue (6): 442-445.doi: 10.3969/j.issn.1004-616x.2011.06.009

• 核辐射安全专家论坛 • Previous Articles     Next Articles

High dose radiation-induced DNA damage using single cell gel electrophoresis

DUAN Zhi-kai，SHI Ai-li，LIU Jian-gong，WANG Bin-sheng，GUO Wan-long，ZHANG Shu-xian   

  1. 1. Radiation Medicine Institute and Environmental Medicine, China Institute for Radiation Protection, Taiyuan 030006; 2. Shanxi Province Rongjun Hospital, Taiyuan 030006, Shanxi, China
  • Received:2011-04-15 Revised:2011-09-29 Online:2011-11-30 Published:2011-11-30
  • Contact: DUAN Zhi-kai

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Abstract: OBJECTIVE: To explore the feasibility of single cell gel electrophoresis (SCGE) in estimation of radiation biological dose. METHODS：Normal peripheral blood samples from two healthy males were exposed to different doses 60Co γ-rays，ranged from 0 to 16 Gy. Immediately and 1 h later，tail moment (TM) of the peripheral blood cells were analyzed with casp software in order to establish dose-effect relationship. RESULTS：The TMs immediately after radiation of peripheral blood cells increased with the irradiation doses，and its relationship can be fitted as：Y＝0.836 7＋0.530 9D＋0.079 9 D2 (R2＝0. 9962，P＜0.05) from 0 to 16 Gy，and Y＝0.400 3＋1.129 1D (R2＝0.9963，P＜0.05) from 0 to 8 Gy. The TMs of peripheral blood cells 1 h after radiation increased with the irradiation doses，and its relationship can be fitted as：Y ＝0.461 6 ＋ 0.656 0D＋0.062 1 D2 (R2＝0.998 4，P＜0.05) from 0 to 16 Gy，and Y＝0.107 1＋1.128 3D (R2＝0.999 5， P＜0.05) from 0 to 8 Gy. CONCLUSION：The TM of peripheral blood cells analyzed with SCGE is a promising radiation biological dosimeter at high doses.

Key words: single cell gel electrophoresis, ionizing radiation, peripheral blood, dose -effect relationship